How to Scale-Up Animal Cell Culture? | Zoology
It is the surface area for cell growth. A unit volume of medium is only capable of giving a finite yield of cells. Factors which affect the yield are: pH, oxygen limitation, accumulation of toxic products (e.g. NH4), nutrient limitation (e.g. glutamine), spatial restrictions, and mechanical/shear stress. As soon as one of these factors comes into effect, the culture is [...]